Dr. Senthilkumar Rajagopal Associate Professor

Specialization

Biochemistry

Areas Of Interest

GPCR signalling in Inflammation and Calcium channels in chronic pain

Qualification

M.Sc., PhD, Post doc

Teaching Experience

17

Awards & Recognition

Vidwan ID/Profile URL

Scopus ID/Google Scholar ID

Funded Project (amount sanctioned)

• Rs. 650000 [Seed Fund - REVA University];
• Rs. 18750 [INSA-NASI-IASc Summer Research fellowship];
• Rs. 6750000 [DBT-Ramalingaswami Re-entry fellowship - Completed]

PhDs Guided/PhDs Guiding

Publications In Refereed Journals

Key Publications (Only Q1 & Q2 Rated)

• Multifarious Beneficial Effect of Nonessential Amino Acid, Glycine: A Review. Razak MA, Begum PS, Viswanath B, Rajagopal S. Oxid Med Cell Longev. 2017;2017:1716701. doi: 10.1155/2017/1716701. Epub 2017 Mar 1. PMID: 28337245
• Inflammatory Mediators in Obesity. Kumar DP, Koka S, Li C, Rajagopal S. Mediators Inflamm. 2019 Jul 31;2019:9481819. doi: 10.1155/2019/9481819. eCollection 2019. PMID: 31485196
• Regulation of Dietary Amino Acids and Voltage-Gated Calcium Channels in Autism Spectrum Disorder. Singh S, Sangam SR, Senthilkumar R. Adv Neurobiol. 2020;24:647-660. doi: 10.1007/978-3-030-30402- 7_24. PMID: 32006378
• Editorial: Regulatory Action of Calcium Channels in Pain Pathway. Rajagopal S, Murugan S, Kumar DP, Kesturu GS, Arul AB. Front Cell Neurosci. 2021 May 31;15:701105. doi: 10.3389/fncel.2021.701105. eCollection 2021. PMID: 34135735
• Modulatory Effects of Dietary Amino Acids on Neurodegenerative Diseases. Rajagopal S, Sangam SR, Singh S, Joginapally VR. Adv Neurobiol. 2016;12:401-14. doi: 10.1007/978-3-319-28383-8_22. PMID: 27651266
• Stimulatory and inhibitory effects of PKC isozymes are mediated by serine/threonine PKC sites of the Cav2.3α1 subunits. Rajagopal S, Burton BK, Fields BL, El IO, Kamatchi GL. Arch Biochem Biophys. 2017 May 1;621:24-30. doi: 10.1016/j.abb.2017.04.002. Epub 2017 Apr 5. PMID: 28389298
• Inhibition of RhoA-dependent pathway and contraction by endogenous hydrogen sulfide in rabbit gastric smooth muscle cells. Nalli AD, Rajagopal S, Mahavadi S, Grider JR, Murthy KS. Am J Physiol Cell Physiol. 2015 Mar 15;308(6):C485-95. doi: 10.1152/ajpcell.00280.2014. Epub 2015 Jan 7. PMID: 25567809
• Contribution of protein kinase Cα in the stimulation of insulin by the down-regulation of Cavβ subunits. Rajagopal S, Fields BL, Kamatchi GL. Endocrine. 2014 Nov;47(2):463-71. doi: 10.1007/s12020-013-0149- y. Epub 2014 Jan 23. PMID: 24452871
• Differential regulation of muscarinic M2 and M3 receptor signaling in gastrointestinal smooth muscle by caveolin-1. Bhattacharya S, Mahavadi S, Al-Shboul O, Rajagopal S, Grider JR, Murthy KS. Am J Physiol Cell Physiol. 2013 Aug 1;305(3):C334-47. doi: 10.1152/ajpcell.00334.2012. Epub 2013 Jun 19. PMID: 23784544
• 10 Activation of transmembrane bile acid receptor TGR5 stimulates insulin secretion in pancreatic β cells. Kumar DP, Rajagopal S, Mahavadi S, Mirshahi F, Grider JR, Murthy KS, Sanyal AJ. Biochem Biophys Res Commun. 2012 Oct 26;427(3):600-5. doi: 10.1016/j.bbrc.2012.09.104. Epub 2012 Sep 27. PMID: 23022524
• Effect of glycine on tissue fatty acid composition in an experimental model of alcohol-induced hepatotoxicity. Senthilkumar R, Nalini N. Clin Exp Pharmacol Physiol. 2004 Jul;31(7):456-61. doi: 10.1111/j.1440-1681.2004.04021.x. PMID: 15236634
• Glycine prevents hepatic fibrosis by preventing the accumulation of collagen in rats with alcoholic liver injury. Senthilkumar R, Nalini N. Pol J Pharmacol. 2004 Jan-Feb;56(1):121-8. PMID: 15047986
• Glycine modulates hepatic lipid accumulation in alcohol-induced liver injury. Senthilkumar R, Viswanathan P, Nalini N. Pol J Pharmacol. 2003 Jul-Aug;55(4):603-11. PMID: 14581719
• Protein kinase C isozyme-specific potentiation of expressed Ca v 2.3 currents by acetyl-beta- methylcholine and phorbol-12-myristate, 13-acetate. Rajagopal S, Fang H, Patanavanich S, Sando JJ, Kamatchi GL. Brain Res. 2008 May 19;1210:1-10. doi: 10.1016/j.brainres.2008.03.017. Epub 2008 Mar 20. PMID: 18420182
• The potential beneficial effect of glycine on the carbohydrate moieties of glycoproteins in an exper- imental model of alcohol-induced hepatotoxicity. Senthilkumar R, Nalini N. J Med Food. 2004 Spring;7(1):108-13. doi: 10.1089/109662004322984798. PMID: 15117562
• Cytokine-induced S-nitrosylation of soluble guanylyl cyclase and expression of phosphodiesterase 1A contribute to dysfunction of longitudinal smooth muscle relaxation. Rajagopal S, Nalli AD, Kumar DP, Bhattacharya S, Hu W, Mahavadi S, Grider JR, Murthy KS. J Pharmacol Exp Ther. 2015 o Mar;352(3):509-18. doi: 10.1124/jpet.114.221929. Epub 2014 Dec 30. PMID: 2555019
• Inhibitory role of Ser-425 of the alpha1 2.2 subunit in the enhancement of Cav 2.2 currents by phor- bol-12-myristate, 13-acetate. Fang H, Patanavanich S, Rajagopal S, Yi X, Gill MS, Sando JJ, Ka- matchi GL. J Biol Chem. 2006 Jul 21;281(29):20011-7. doi: 10.1074/jbc.M601776200. Epub 2006 May 16. PMID: 16704976
• Activation of G protein-coupled bile acid receptor, TGR5, induces smooth muscle relaxation via both Epac- and PKA-mediated inhibition of RhoA/Rho kinase pathway. Rajagopal S, Kumar DP, Mahavadi S, Bhattacharya S, Zhou R, Corvera CU, Bunnett NW, Grider JR, Murthy KS. Am J Phys- iol Gastrointest Liver Physiol. 2013 Mar 1;304(5):G527-35. doi: 10.1152/ajpgi.00388.2012. Epub 2012 Dec 28. PMID: 23275618
• Formalin-induced short- and long-term modulation of Cav currents expressed in Xenopus oocytes: an in vitro cellular model for formalin-induced pain. Rajagopal S, Fang H, Lynch C 3rd, Kamatchi GL. Basic Clin Pharmacol Toxicol. 2010 Apr;106(4):338-47. doi: 10.1111/j.1742- 7843.2009.00496.x. Epub 2009 Dec 22. PMID: 20030632
• Release of GLP-1 and PYY in response to the activation of G protein-coupled bile acid receptor TGR5 is mediated by Epac/PLC-ε pathway and modulated by endogenous H2S. Bala V, Rajagopal S, Kumar DP, Nalli AD, Mahavadi S, Sanyal AJ, Grider JR, Murthy KS. Front Physiol. 2014 Nov 3;5:420. doi: 10.3389/fphys.2014.00420. eCollection 2014. PMID: 25404917
• Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Dhandapani S, Subramanian VR, Rajagopal S, Namasivayam N. Pharmacol Res. 2002 Sep;46(3):251-5. doi: 10.1016/s1043-6618(02)00131-7. PMID: 12220968

Other Publications

• B. Manimegalai, GV. Swarnalatha, Veerappan Ramanathan, Senthilkumar Rajagopal. Effect of Achyranthes Aspera (Linn) seeds on membrane-bound ATPases in selected tissues of rats fed with high doses of fructose. Current Trends in Biotechnology and Pharmacy. 2021; 15 (4): 371-377.
• Veerappan Ramanathan, Swarnalatha GV, Senthilkumar Rajagopal. Suppression of hepatic oxida- tive events and regulation of eNOS expression in the liver by chrysin in high fructose-fed rats. Chet- tinad Health City Medical Journal. 2021; 10 (1): 1-7.
• Veerappan Ramanathan, GV Swarnalatha, B. Manimegalai, A. Dominic Amalraj, Senthilkumar Rajagopal. Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting en- zymes in L-NAME induced hypertensive rats. Current Trends in Biotechnology and Pharmacy. 2020; 14 (3): 327-339.
• Pathan Shajahan Begum, Meerza Abdul Razak, Senthilkumar Rajagopal, Vakita Venkataratnam- ma. Antiviral Effects Of Probiotic Bacteria Isolated From Fresh Water Fishes Against White Spot Syndrome Virus. Journal of Emerging Technologies and Innovative Research. 2018; 5(11): 76-91.
• Pathan Shajahan Begum, Meerza Abdul Razak, Senthilkumar Rajagopal, V. Venkataratnamma. Effect of probiotic bacteria on shrimp pond ecosystem and their influence on growth and survival of Litopenaeus Vannamei. International Journal of Scientific Research in Sci- ence, Engineering and Technology. 2018; 4(4): 1158-1165.
• Syeda Kulsum, G. Vidya Sagar, R.Senthilkumar, Syed Mohammed Kazim. Bio-analytical method development and validation for metformin and canagliflozin by RP-HPLC. World Journal of Phar- macy and Pharmaceutical Science. 2017; 6(12): 371-381.
• Veerappan Ramanathan, Senthilkumar Rajagopal. Chrysin ameliorates the lipid profiles in Nω- nitro-l-arginine-methylester-induced hypertensive rats. American Journal of Biochemistry and Mo- lecular Biology; 2016; 6: 60-66.
• MA. Razak, S. Rajagopal, PA. Basha, DM. Rao, PS. Begum, D. Rajakumari, B. Viswanath. En- hancing the activity aspartate kinase for an overproduction of L-lysine by Corynebacterium glu- tamicum. American Journal of Biochemistry and Molecular Biology; 2016; 6:33-44.
• Senthilkumar Rajagopal, Blanche L. Fields and Ganesan L. Kamatchi. Contribution of Protein Kinase C Isozyme in the Stimulation of Insulin Secretion by the Inhibition of Cavβ subunits. En- docrine; 2014; 47(2):463-471.
• Vanitha Bala, Senthilkumar Rajagopal, Divya P. Kumar, Sunila Mahavadi, Ruizhe Zhou, Zachary L. Bradley, Nigel W Bunnett, Carlos U. Corvera, Johan Auwerx, John R. Grider, Arun J. Sanyal, Karnam S. Murthy. Hydrogen Sulfide (H2S) Inhibits Bile Acid Receptor TGR5-Mediated GLP-1 Release from Enteroendrocrine Cells: Possible Involvement of H2S in Altered Glucose Metabolism in Diabetes. Gastroenterology. 2012; 142 (5): S-50.
• Vanitha Bala, Sunila Mahavadi, Senthilkumar Rajagopal, Ruizhe Zhou, John F. Kuemmerle, Arun J. Sanyal, Karnam S. Murthy. Bile Acid-Induced Stimulation of ERK1/2 Activity, GLP-1 and PYY Release in Enteroendocrine Cells are mediated by the Activation of Epac/PLC-ɛ Signaling Pathway via GS-Coupled TGR5. Gastroenterology. 2011; 140 (5): S-147.
• Senthilkumar Rajagopal, Othman Al-Shboul, Sunila Mahavadi, John F Kuemmerle, Karnam S Murthy. Expression and Function of Bile Acid Receptor TGR5 in Gastrointestinal Smooth Muscle. FASEB Journal, 2011; 25: 1069.5.
• Othman Al-Shboul, Sunila Mahavadi, Senthilkumar Rajagopal, Ruizhe Zhou, Karnam S Murthy. Differences in the Expression of VPAC2 Receptors and Adenylyl Cyclase V/VI and VIP-Induced cAMP Generation in Phasic and Tonic Smooth Muscles. FASEB Journal, 2011; 25: 1069.7.
• Senthilkumar Rajagopal, Hongyu Fang, Carl Lynch III, Ganesan Kamatchi L. Formalin -induced –short and long-term modulation of Cav currents expressed in Xenopus oocytes: An in vitro cellular model for formalin –induced pain. Basic and Clinical Pharmacology and Toxicology. 2010; 106: 338-347.
• M.Sengottuvelan, R.Senthilkumar, N.Nalini. Modulatory influence of dietary resveratrol during different phases of a one, 2-dimethylhydrazine induced mucosal lipid-peroxidation, antioxidant sta- tus and aberrant crypt foci development in rat colon carcinogenesis. Biochimica Biophysica Acta. 2006; 1760: 1175-1183.
• R. Senthilkumar, N. Nalini. The potential beneficial effect of glycine on the carbohydrate moieties of glycoproteins in an experimental model of alcohol induced hepatotoxicity. Journal of Medicinal Food. 2004, Spring; 7(1): 108-113.
• R. Senthilkumar, P. Viswanathan, N. Nalini. Effect of glycine on oxidative stress in rats with alco- hol induced liver injury. Die Pharmazie. 2004; 59(1): 55-60.
• R.Senthilkumar, M.Sengottuvelan, N. Nalini. Protective effect of glycine supplementation on the levels of lipid peroxidation and antioxidant enzymes in the erythrocyte of rats with alcohol induced liver injury. Cell Biochemistry and Function. 2004; 22(2): 123-128.
• Pathan Shajahan Begum, Gujjula Madhavi, Senthilkumar Rajagopal, Buddolla Viswanath, Meer- za Abdul Razak, V. Venkataratnamma. Probiotics as functional foods: potential effects on human health and neurological diseases. International Journal of Nutrition, Pharmacology, Neurological Diseases. 2017; 7:23-33.
• Senthilkumar Rajagopal, Shubham Singh, Supraj Raja Sangam. Contribution of Protein Kinase C (PKC) in the modulation of Voltage gated Calcium Channels. Journal of Pharmaceutical and Bio- medical Sciences. 2015; 5(4):351-354.

Research Summary

In our study, we demonstrate that in gastric smooth muscle, TGR5 receptors are coupled to Gs/cAMP pathway and activation of these receptors leads to muscle relaxation via both PKA- dependent phosphorylation of RhoA, and PKA-independent, Epac-dependent stimulation of Rap1. TGR5 receptor activation on smooth muscle reveals a novel mechanism for the regulation of gut motility by bile acids.

The progression of alcoholic liver disease is characterized by steatosis, inflammation, necrosis and finally fibrosis and cirrhosis. When severe hepatitis occurs, death is outcome. Our study was aim to evaluate the hepatoprotective effect of glycine, a non-essential amino acid in alcohol-induction.

International Visits

• USA
• Canada

Patents Copyrights

Books Published

UG / PG Projects